igepal ca360 (Thermo Fisher)
Structured Review
Igepal Ca360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igepal+ca+630/IGEPAL%26mid%3Br+CA-630/10__1016_slash_j__apsb__2026__06__031-205-26-34
Average 95 stars, based on 1 article reviews
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Protease Inhibitor:Article Title: LAMP1 and LAMP2A localise to axonal organelles with distinct motility dynamics and partially overlapping molecular signatures in human neurons Article Snippet: .. Cells were lysed in 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 1% Article Title: Pathogenic PTCD1 variants cause mitochondrial protein aggregation and cardiomyopathy Article Snippet: .. Each sample was resuspended in 0.5 mL of RIPA buffer containing 1% Article Title: Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli Article Snippet: .. Briefly, cell pellets were resuspended in 1/20 culture volume of lysis buffer (50 mM Tris at pH 7.9, 100 mM KCl, 0.5% [w/v] Extraction:Article Title: Durable protection against SIV challenge by adeno-associated virus delivery of Env-specific antibodies Article Snippet: .. Upon thawing on ice, the adhered material was manually homogenized with the weck itself in the same tube with 0.3 ml of the freshly prepared ice-cold extraction buffer consisting of 1X PBS, 2 mM EDTA, 0.5% Incubation:Article Title: Durable protection against SIV challenge by adeno-associated virus delivery of Env-specific antibodies Article Snippet: .. Upon thawing on ice, the adhered material was manually homogenized with the weck itself in the same tube with 0.3 ml of the freshly prepared ice-cold extraction buffer consisting of 1X PBS, 2 mM EDTA, 0.5% Article Title: Pathogenic PTCD1 variants cause mitochondrial protein aggregation and cardiomyopathy Article Snippet: .. Each sample was resuspended in 0.5 mL of RIPA buffer containing 1% Isolation:Article Title: Single-molecule dynamics of the TRiC chaperonin system in vivo. Article Snippet: .. Lysate was digested with 500 U per 1 mg RNase I (Lucigen) at 4 °C for 1 h. Ribosome pellets were isolated using a 25% sucrose cushion prepared in lysis buffer without Lysis:Article Title: Single-molecule dynamics of the TRiC chaperonin system in vivo. Article Snippet: .. Lysate was digested with 500 U per 1 mg RNase I (Lucigen) at 4 °C for 1 h. Ribosome pellets were isolated using a 25% sucrose cushion prepared in lysis buffer without Article Title: Substitution of the SEMA3A basic domain with the VEGF 165 heparin-binding domain enhances anti-angiogenic potency in ovo. Article Snippet: .. After the last washing step, the beads were resuspended into 4x Laemmli buffer and protein complexes were denatured by heating samples at 95°C for 5 min. For whole-cell extract (WCE) preparation, transfected cells were lysed in RIPA lysis buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 1% Article Title: Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli Article Snippet: .. Briefly, cell pellets were resuspended in 1/20 culture volume of lysis buffer (50 mM Tris at pH 7.9, 100 mM KCl, 0.5% [w/v] Transfection:Article Title: Substitution of the SEMA3A basic domain with the VEGF 165 heparin-binding domain enhances anti-angiogenic potency in ovo. Article Snippet: .. After the last washing step, the beads were resuspended into 4x Laemmli buffer and protein complexes were denatured by heating samples at 95°C for 5 min. For whole-cell extract (WCE) preparation, transfected cells were lysed in RIPA lysis buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 1% Sonication:Article Title: Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli Article Snippet: .. Briefly, cell pellets were resuspended in 1/20 culture volume of lysis buffer (50 mM Tris at pH 7.9, 100 mM KCl, 0.5% [w/v] Sedimentation:Article Title: Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli Article Snippet: .. Briefly, cell pellets were resuspended in 1/20 culture volume of lysis buffer (50 mM Tris at pH 7.9, 100 mM KCl, 0.5% [w/v] |
